| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff81a650b70e913d3b2524b45e4c7320 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-117
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L2430-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-90 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3695
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3633
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0677 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-14 |
| filingDate |
2017-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd0b8c8c450e14162f38aac7a1494abc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b604e7110c707d6b50df69a4211e7938
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_899dd1dab422a317852b0b1de4d59f8c |
| publicationDate |
2020-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10767164-B2 |
| titleOfInvention |
Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| abstract |
Human pluripotent stem cells (hPSCs) are promising cell source to produce therapeutic endocrine cells for diabetes treatment. A gel solution made by decellularized tissue-specific extracellular matrix (dpECM) significantly promotes three-dimensional (3D) islet-like organogenesis during induced hPSC differentiation into endocrine lineages. Islet organoids are self-organized even in a two-dimensional (2D) culture mode. Cells derived from hPSCs differentiated on such ECM coated substrates exhibit similar cellular composition to native pancreatic islets. These cells express islet signature markers insulin, PDX-1, C-peptide, MafA, glucagon, somatostatin, and pancreatic polypeptide, and secrete more insulin in response to glucose level compared to a traditional matrix substrate (Matrigel). The dpECM facilitates generating more C-peptide+/glucagon− cells rather than C-peptide+/glucagon+ cells. Remarkably, dpECM also facilitated intra-organoid vascularity by generating endothelial cells and pericytes. Furthermore, dpECM niches also induced intra-organoid microvascularization during pancreatic differentiation. |
| priorityDate |
2017-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |