| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42cb705529d61d486910ef44f32d36ac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202b536b4560d4a4ac8c0d21415d3fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_716e9a2606ffd4ba1a2a3917e4a402fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95979765e90bc2698303119c70553317
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_970e454d34bdfe33ac2605d85713e720 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-28 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0677 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate |
2011-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6fc223deb914a93dba830ebca980b380
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21b5cea9b9a2654949fd7423027b0ad1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c40baf596c2e4b393d485c57937cc49
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_965b76e5524eb5f4912e0c89b7f11f4b |
| publicationDate |
2013-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2013122586-A1 |
| titleOfInvention |
Method of culturing pancreatic islet-like tissues by a tissue complex of pancreas-derived non-endocrinal epithelial cells and vascular endothelial cells |
| abstract |
According to embodiments, a method of producing insulin-producing tissues (IPTs) by culturing comprises: preparing non-endocrinal epithelial cells (NEECs) and vascular endothelial cells (VECs), which have been isolated or originated from postnatal pancreata, preferably by capturing of NEECs by collagen; culturing in vitro the NEECs and the VECs at least partly separately from each other; and then generating in vitro a tissue complex (IPTs) that contains both the NEECs and the VECs. In another embodiment, the native islet cells are seeded on a monolayer of VECs that have preferably been separately cultured and purified. In a further embodiment, a method of enriching NEECs comprises: culturing NEECs adhering to a container or substrate; removing NEECs by treating with a tissue-dissociation enzyme to leave left-over cells (LOCs) still attached on the container or substrate; and culturing NEECs in a medium conditioned by, or in the presence of the LOCs. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10799615-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10767164-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016179106-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11466255-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017214465-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016017288-A1 |
| priorityDate |
2010-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |