| abstract |
The invention is a method for generating nucleic acid sequences ends which comprises; n (a) hybridizing a primer to a nucleic acid sequence, n (b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and n (c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence. n The invention provides a method for generating target nucleic acid sequences for subsequent amplification. The method is applicable to both DNA and RNA. |