Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f00bf87e88cbeb47a4095e408abba00e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5c1d546e69701ddc4519cd9b4bee1a38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45a04614e69114d4ba1c994a676d8619 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e1e5f2fd5b25f1b5475dc433edf61c24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c0c315015a0ed6d5e494a0f2e4028a5e |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-607 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6846 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2001-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2003-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a2d12181fb534d06dced3f16011a857 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc1fbc7500dae90bb512e77b6c79d3b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eebfad12d39cccd85217b0969579affa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f252f42d41038e0ec2860fff6abe37eb |
publicationDate |
2003-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6589742-B2 |
titleOfInvention |
Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures |
abstract |
A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8703445-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006188880-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006110754-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9752182-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010330575-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2418018-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8048633-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7829275-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010297708-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005136441-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010000881-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8017340-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010167281-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005191651-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8883487-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004180369-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8580577-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003143581-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003219804-A1 |
priorityDate |
1999-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |