Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f9476149a720249ab288c591564fdf3 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
filingDate |
1999-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2000-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8af2f553ac631359fc79bc80689ff281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eadde8c853ce5be7b9cb105b64cf23e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a5563b268902f18f71d175d0635e29c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_060611962dab6bb0d02d0cda5c27c77b |
publicationDate |
2000-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-6130047-A |
titleOfInvention |
Detection of nucleic acids by fluorescence quenching |
abstract |
Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target. In the presence of target, however, the second oligonucleotide(s) of the detector nucleic acid is/are completely or partially displaced from the first, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence which may be detected as an indication of the presence of the target sequence. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2110439-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003082713-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11214825-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8323929-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450599-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007026423-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004011908-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007184453-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010021901-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009246792-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004092412-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005042618-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03068918-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3464592-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10815519-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009131647-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7169554-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003219788-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11168357-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9834439-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7005265-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006119326-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006119326-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03068918-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10480030-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003165913-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9856472-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6815164-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6379888-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7252937-B2 |
priorityDate |
1997-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |