| abstract |
PCT No. PCT/EP88/00024 Sec. 371 Date Aug. 18, 1988 Sec. 102(e) Date Aug. 18, 1988 PCT Filed Jan. 14, 1988 PCT Pub. No. WO88/05434 PCT Pub. Date Jul. 28, 1988.The invention relates to D-aminoluciferin derivatives of the general formula I <IMAGE> (I) wherein R1 represents a L-amino acid or peptide radical having up to 10 L-amino acid moieties said radical being bound via the (terminal) carboxyl group in the form of an amide and the free amino group thereof is optionally be protected by a common protecting group, or wherein R1 represents a monosaccharide or disaccharide radical. Said aminoluciferin derivatives can be cleaved by enzymes giving free aminoluciferin which can be determined luminometrically. The invention relates also to the use of said aminoluciferin derivatives for determining enzyme activities as well to a process for producing said aminoluciferin derivatives. |