| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0db30c27e36aa956e09406528b2e26d0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb28dd172d2102eb407e005f1af8f891
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_19ce92c4b17181190e1f0524cd648498
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_38ae2322fafdb332823ff6bd3d2ef30c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34d3488e09b022161a04307f9d592148
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e1a9378dd768f8995e23bdded9a4c79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b59c8ba90384bd76668d848027a97e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa93eaff1f07b1230108e7c9e8c11de6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527a34d5db056642ca8c004bd37df32e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2cab9b3bd32a97b9d7925e27bf8d8854 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate |
2010-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dde642f52d41905cc8a93576e42d902c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e92c31548518642ee286768bb88803c5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8985b9d29a6d562a6fafb821d883e1d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_559e26ee99a602f4fd0324b8d43c17fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c38b5130dab5f04ca46374a8556a5bdd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1bba3faa0cf200b229dc706b64ef104
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbe1698267f64763292ea6eea6127e20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_025ee916d867ef48eb46818187914ea1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc709c8493e55241613fc67bc1fd53be |
| publicationDate |
2012-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2012013204-A1 |
| titleOfInvention |
Novel ultrasensitive cell based sensors and uses thereof |
| abstract |
The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013113370-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022258997-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10913974-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016538823-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11254971-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9499584-B2 |
| priorityDate |
2010-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |