abstract |
A method of preparing a 6-substituted D-luciferin ester suitable for use in the determination of pesticides by a bioluminescence method. The 6-substituted D-luciferin esters, such as 6-D-luciferin acetate, are prepared in a liquid solvent containing N-acetyl imidazole with D-luciferin and recovering as the reaction product 6-D-luciferin acetate. |