| abstract |
A CRISPR/Cas9 gene editing system and application thereof. The gene editing system is a complex formed by a specific Cas9 protein and sgRNA, and can precisely position a target DNA sequence and cause cutting so that double strand breaks occur to the target DNA sequence; the gene editing refers to intracellular or in vitro gene editing. The used specific Cas9 protein is small and only has about 1000 amino acids, and thus an identified PAM sequence is simple; the Cas9 protein has an amino acid sequence represented by any one of SEQ ID NOs: 1-10 and 58; the sgRNA has a nucleotide sequence represented by SEQ ID NO: 11. |