http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110577969-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110577969-B |
titleOfInvention | CRISPR/Sa-SlugCas9 gene editing system and its application |
abstract | The invention belongs to the technical field of gene editing, in particular to a CRISPR/ Sa-SlugCas9 gene editing system and an application thereof. The gene editing system of the present invention is a complex formed by Sa-SlugCas9 protein and sgRNA, which can precisely target DNA sequence and generate cutting to cause double-strand break damage to DNA; the gene editing is gene editing in cells or in vitro; Sa-SlugCas9 is a fusion protein, which replaces the PAM recognition domain (PAM interacting, PI) of SaCas9 with the PAM recognition domain (SlugCas9-PI) of SlugCas9. The Sa-SlugCas9 protein is small, 1055 amino acids, and the recognized PAM sequence is simple. The Sa -SlugCas9 protein has the amino acid sequence shown in SEQ ID NO: 1, and the sgRNA has the nucleotide shown in SEQ ID NO: 2 sequence. The invention has broad application prospects in the field of gene editing. |
priorityDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 455.