abstract |
The invention belongs to the technical field of gene editing, in particular to a CRISPR/ Sa-SepCas9 gene editing system and its application. The gene editing system of the present invention is a complex formed by Sa-SepCas9 protein and sgRNA, which can precisely target DNA sequences and generate cleavage to cause DNA double-strand break damage; the gene editing is gene editing in cells or in vitro; Sa-SepCas9 is a fusion protein, replacing the PAM recognition domain (PAM interacting, PI) of SaCas9 with the PAM recognition domain of SepCas9 (SepCas9-PI). The Sa-SepCas9 protein is small, with 1055 amino acids, and the recognized PAM sequence is simple. The Sa-SepCas9 protein has the amino acid sequence shown in SEQ ID NO: 1, and the sgRNA has the nucleotide shown in SEQ ID NO: 2 sequence. The invention has broad application prospects in the field of gene editing. |