| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90010bd244bf98367bc08972999b3d41 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-179
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-514
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-161 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y306-01001 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate |
2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3710161f3a3c6b3a58d646148570f1e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_499f34b16f73eef80ef28e6282749042
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8249da359604839187dbb87126698925
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c5fcb14032c3fe22663797f3e2236e9 |
| publicationDate |
2020-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2020016590-A1 |
| titleOfInvention |
Improved polynucleotide sequence detection method |
| abstract |
A method of detecting a target polynucleotide sequence in a given nucleic acid analyte characterised by the steps of: a. annealing the analyte to a single-stranded probe oligonucleotide A0 to create a first intermediate product which is at least partially double-stranded and in which the 3' end of A0 forms a double-stranded complex with the analyte target sequence; b. pyrophosphorolysing the first intermediate product with a pyrophosphorolysing enzyme in the 3'-5' direction from the 3' end of A0 to create partially digested strand A1 and the analyte; c. (i) annealing A1 to a single-stranded trigger oligonucleotide B and extending the A1 strand in the 5'-3' direction against B; or (ii) circularising A1 through ligation of its 3' and 5' ends; or (iii) ligating the 3' end of A1 to the 5' end of a ligation probe oligonucleotide C; in each case to create an oligonucleotide A2; d. priming A2 with at least one single-stranded primer oligonucleotide and creating multiple copies of A2, or a region of A2; and e. detecting a signal derived from the multiple copies and inferring therefrom the presence or absence of the polynucleotide target sequence in the analyte. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022136875-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11332780-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021130494-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021130496-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022269275-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11634754-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10961569-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022175655-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022175657-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3842549-A1 |
| priorityDate |
2018-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |