http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022269275-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90010bd244bf98367bc08972999b3d41 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate | 2022-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b99dc13d16bd262ecfd79be49f4908f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_499f34b16f73eef80ef28e6282749042 |
| publicationDate | 2022-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022269275-A1 |
| titleOfInvention | Polynucleotide detection |
| abstract | This invention relates to a method of detecting two or more target polynucleotide sequences comprising using a probe oligonucleotide A0 annealing to the target, whereafter the 3' end is pyrophosphorolysed and the remaining probe is circularised or ligated to another probe using a splint oligonucleotide, and a padlock probe X0, which is circularised against the target and not pyrophosphorolysed, and then a signal from (amplicons of) the ligated probes is detected to indicate presence of the target. Further disclosed are kits comprising the probes and enzymes as well as buffer and a source of pyrophosphate ions. |
| priorityDate | 2021-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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