http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018080074-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b5d47c4f1d77d82de7ce1f64ba985b3 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-319 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-629 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-159 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-162 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2017-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fa5e6e5c90e8f185648f8fbe551d30f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28f845b0c76a27e4f9f4e2224ffda0cd |
publicationDate | 2018-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2018080074-A1 |
titleOfInvention | Single nucleotide detection method |
abstract | A method of sequencing a nucleic acid such as DNA or RNA is provided. It is characterised by the steps of (1) generating a stream of single nucleoside triphosphates by progressive pyrophosphorolysis of the nucleic acid; (2) producing at least one substantially double-stranded oligonucleotide used probe by reacting in the presence of a polymerase and a ligase at least one of the single nucleoside triphosphates with a corresponding probe system comprising (a) a first single-stranded oligonucleotide labelled with characteristic detectable elements in an undetectable state and (b) second and third single-stranded oligonucleotides capable of hybridising to complementary regions on the first oligonucleotide; (3) digesting the used probe with an enzyme having double-stranded exonucleolytic activity to yield the detectable elements in a detectable state and a single-stranded fourth oligonucleotide which is at least in part the sequence complement of the first oligonucleotide; (4) reacting the fourth oligonucleotide with another first oligonucleotide to produce a substantially double-stranded oligonucleotide product corresponding to the used probe; (5) repeating steps (3) and (4) in a cycle and (6) detecting the characteristic detectable elements released in each iteration of step (3). Suitably the detectable elements are fluorophores. The method of the present invention generates a stronger fluorescence signal from a single nucleoside triphosphate than has been described previously. Suitable probe systems are also disclosed. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020016590-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112424379-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10961569-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11332780-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3889273-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102589502-B1 |
priorityDate | 2014-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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