| abstract |
The invention is concerned with methods for remodeling cell chromatin and with methods of obtaining pluripotent, multipotent and/or unipotent cells. The methods comprise contacting somatic cells with at least one polynucleotide encoding an oocyte-deprogramming polypeptide. Expression of an oocyte-deprogramming polypeptide in the somatic cell modifyies the DNA of the somatic cell, modifies the chromatin of the somatic cell and/or remodels its chromatin. Also described are methods for deprogramming a somatic cell, and methods for conditioning somatic cells to differentiation into a pluripotent, multipotent or unipotent cell. In addition, the invention encompasses cells and cell lines obtained according to these different methods. |