| abstract |
The present invention addresses the problems of: providing a method for producing motor neurons from pluripotent stem cells quickly and in synchronisation, said motor neurons being satisfactorily provided with the properties of inherent motor neurons (particularly in patients suffering from a disease); and providing the pluripotent stem cells, which are capable, by a chemical treatment, of differentiating quickly and in synchronisation into the motor neurons that are satisfactorily provided with the properties of the inherent motor neurons. The method for producing motor neurons from pluripotent stem cells includes steps (1)-(2) in the stated order: (1) a step for introducing into the pluripotent stem cells a nucleic acid that encodes Lhx3, Ngn2, and Isl1; and (2) a step for maintaining the expression of Lhx3, Ngn2, and Isl1 for three days or more. |