| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c91bb38326c9ad10a98bbf2cbd56bc6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a88b8ec09e31a28046ea82a2ee8b3e72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e6820fb11535380870c57d507b020c40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c30463cb29dc2b646a28c8e1d63cf90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7e1c04975c9146a64d93c0606c121ab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8769b1f5d208ea9906d36eff78c9104
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b20036e5e1bf09a9a6a9a98ff905d5a6 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate |
2007-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33111258e9eb700fdd60c6b6ad06ad35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_421ef97fcf22662971d6778117d991c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9bb002461ec133fc30d13a9849fa09c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0422f1bc5fb26f2f78a4145732893425
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99336cd1529de725986c371b7673a66b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da33aa1f37a032fed5f5004038b31f97 |
| publicationDate |
2007-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2007135354-A1 |
| titleOfInvention |
Improved uracil-excision based molecular cloning |
| abstract |
The invention provides an improvement to a uracil-specific excision reagent (USER™) based cloning technique comprising generating a single-stranded extension on a polynucleotide molecule, the single-stranded extension having a desired length and sequence composition, comprising: (a) introducing a cassette into a polynucleotide molecule at a predetermined location; (b) cleaving the polynucleotide molecule with a nicking endonuclease specific for a nicking site in the cassette and with a restriction endonuclease specific for a restriction site in the cassette; and (c) dissociating the cleaved polynucleotide molecule between the nicking site and the restriction site to generate the single-strand extension with the desired length and sequence composition; wherein said improvement to said USER™ based cloning technique comprises using a proof-reading polymerase to generate said polynucleotide molecule. Also provided is a deoxyuridine-excision based method of fusing duplex nucleic acid sequence to form a fused duplex product referred to herein as 'USER™ fusion'. The invention further provides related materials and methods. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109439682-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015103741-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009000281-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10837012-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113227370-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020135651-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017046594-A1 |
| priorityDate |
2006-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |