| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c56fe4745971a33df7d9e30e3d477cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527a34d5db056642ca8c004bd37df32e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dabdf917e2432e32e0338355c06da166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_407334914986e5da975293dae6992a50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f48cb2ae86a40b6687b52e7a66e17797
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_68470742c37db68ce35b8ae425ce50cc |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2007-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3829581a701d2eb99be5a8090259dbc1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3486d9ad300c46a159bba8f9e2601928
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c95be12fdcf0a28b961dda76143912be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c7391e822cc66d4d813dbce379c2859
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4bcff43da8442bc6b5c911ea9187f7e |
| publicationDate |
2008-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009000281-A1 |
| titleOfInvention |
Methods for directional cloning of amplified nucleic acids and uses thereof |
| abstract |
Methods are described for producing recombinant DNA molecules from suitable host vectors digested with restriction endonucleases and target nucleic acids subjected to amplification with primers comprising nicking enzyme recognition sequences and nicking enzyme digestion that produce protruding ends in the target nucleic acid. This method takes advantage of the low melting point of the base(s) that results from nicking enzyme treatment at the end of amplified target nucleic acids. Those base (s) when released from the double strand end, produce a compatible end with a vector digested with a properly selected restriction endonuclease, that can be ligated and transformed in a compatible host to obtain a directionally cloned recombinant DNA molecule. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107012205-A |
| priorityDate |
2007-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |