| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a101d4e74a6ab509b4c4864330a6e12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d559a6de6f7c2e4ce2459fb5d17e2b28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0efb5083f0a546e81b40dcaa115ace56 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_989e25b86ff612bdbfc551b22b838c3b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f58124ade917e1123d85e4fd9605e30a |
| publicationDate |
2015-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2015103741-A1 |
| titleOfInvention |
Efficient gene cloning method and uses thereof |
| abstract |
Provided are methods and compositions for molecular cloning of DNA. The methods comprise preparing DNA fragments with sticky ends using a primer design method based on nicking endonucleases and ligating the DNA fragments with cloning vectors that have been engineered to have sticky ends that are complementary to those of the DNA fragments. The methods are highly efficient and can be used to clone fragments of DNA up to 12 Kb. The methods also allow one-step ligation of multiple DNA fragments in a designed orientation. Also provided are commercial gene cloning kits. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11613777-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106047912-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018039599-A1 |
| priorityDate |
2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |