| abstract |
It is intended to provide a polynucleotide which exerts a high RNA interfere effect on a target gene but has little risk of causing RNA interfere on genes not relating to the target gene. From the base sequence of a target gene of RNA interfere, a sequence part following the rules (a) to (d) is detected and, based on the detection results, a polynucleotide causing RNAi is designed, synthesized and so on. (a) The 3’-terminal base is adenine, thymine or uracil. (b) The 5’-terminal base is guanine or cytosine. (c) The 3’-terminal sequence consisting of seven bases is rich in one or more bases selected from the group consistingof adenine, thymine and uracil. (d) The base number is at such a level as causing RNA interfere without expressing cytotoxicity. |