| abstract |
The invention relates to the fields of biochemistry, molecular biology, and pharmacology. More specifically the present invention relates to the production of proteins in a (host) cell. The present invention uses a TRAnscription Pause (TRAP) sequence to enhance a protein expression characteristic of a protein expression unit. The TRAP sequence is thought to at least in part prevent formation of antisense RNA or to at least in part prevent transcription to enter said protein expression unit. In one embodiment, the invention provides a method for expression of at least one protein of interest in a cell comprising providing said cell with at least one protein expression unit which unit comprises a promoter functionally linked to an open reading frame encoding said at least one protein of interest, characterised in that said protein expression unit further comprises at least one TRAnscription Pause (TRAP) sequence and wherein said TRAP sequence is functionally located downstream of said open reading frame and at least in part prevents formation of antisense RNA. In yet another embodiment said TRAP sequence is functionally located upstream of said promoter and at least in part prevents transcription to enter said expression unit. Preferably, the expression protein unit further comprises at least one STabilizing Anti-Repressor (STAR) sequence. |