| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_964276dac951e621ae89e88d9ab5029c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_296106c741f3de3e95f822012050e750
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bea1e77dcc7055b9cd13cc09ec6c83f9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50135123ba50354d7b10cf6830d48928
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb705a4797e6d188fec510a4b5614018 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-46 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate |
2007-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_526b621e5a56fc670c5d1a6bfcb952ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79240c335633f0503e0a27f97b3872a3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f5204f08d34897e10080dde58b62f57
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f5c897bca2bc79773d75c0e4bf63a34 |
| publicationDate |
2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8999667-B2 |
| titleOfInvention |
Selection of host cells expressing protein at high levels |
| abstract |
The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in the multicistronic transcription unit, and wherein an internal ribosome entry site is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG start codon. Also provided are methods for obtaining host cells expressing a polypeptide of interest, such host cells comprising DNA molecules of the invention. Further provided is the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules of the invention. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11015059-B2 |
| priorityDate |
2004-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |