| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50ff049630d6fdb55e58e423057d7e12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa65c116cbd7d0703749e01f895d7720
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82c877b89b6a9adf06cc21ca9943d46d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_94f6038596f5a809d67d8ea0fca2000a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c123a954414370f8e9cd69e568fe92f3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-746
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2001-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cacc66860e7248661b9b0e3779c8d355
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d3d5eda951ce3b6dbf91de2f75b55f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec22b5a88d3c9674b44e989bdeaed038
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bce2ee68b375080e4bcf4d8ed7c0d6a9 |
| publicationDate |
2001-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0162944-A2 |
| titleOfInvention |
Intergenic and intragenic integration sites for foreign gene expression in recombinant s. gordonii strains |
| abstract |
The present invention provides two new chromosomal integration sites for expression of foreign genes have been developed in Streptococcus gordonii (S. gordonii). One integration site is intergenic between orfA and orfB in an operon of unknown function. The other site is intragenic within the lacG gene, which encodes phospho- β-galactosidase, and is part of the lactose (lac) operon. Them6 gene from Streptococcus pyogenes was integrated in a stable configuration into the chromosome of S. gordonii at each of these integration sites, and in both cases the recombinant bacteria expressed the M6 protein on their surface. Furthermore, expression from the lacG site within the lactose operon was shown to be regulated by growth on lactose. Identification of these new chromosomal insertion sites provides the ability to express multiple foreign genes from the same recombinant and the potential for modulating expression in vitro or in vivo by the use of a biosynthetic metabolite. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2012264589-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9920324-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107267546-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10905727-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103562390-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11786567-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103562390-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3360967-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021097671-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014518062-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017200477-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10988770-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2644346-C2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140027405-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012164083-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102066292-B1 |
| priorityDate |
2000-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |