| abstract |
The present invention provides a pharmaceutical composition, comprising a first non-infectious, non-integrating nucleic acid molecule construct comprising a polynucleotide encoding a Flt-3 ligand, and a second non-infectious, non-integrating nucleic acid molecule construct comprising a polynucleotide encoding an antigen or a cytokine. The present invention also provides a method broadly directed to enhancing immune response of a vertebrate in need of immunotherapy which entails administering in vivo, into a tissue of a vertebrate, a Flt-3 ligand-encoding polynucleotide and one or more antigen- or cytokine-encoding polynucleotides. The polynucleotides are incorporated into the cells of the vertebrate in vivo, and a prophylactically or therapeutically effective amount of a Flt-3 ligand and one or more antigens is produced in vivo. |