http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9650606-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a3721ca264753e2b424149847dc94df |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1335 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0658 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate | 2014-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b3729e9f08f73dc52f3bc9636c1f1ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75b581051e293f10874e91041f222462 |
| publicationDate | 2017-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9650606-B2 |
| titleOfInvention | Method of co-culturing mammalian muscle cells and motoneurons |
| abstract | The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11022605-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11613732-B2 |
| priorityDate | 2009-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 664.