http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8664355-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_958fe5e7f345ca0a091332d5afd38538 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba991b44b7fc068de6e10ba552afb5ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8e9ec6bcff60f024dd514391796c689 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-00 |
| filingDate | 2007-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0ee29f8078cd1713439c974078a6fa3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac98494763dfca36566c639704db680b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8efe89061c5abfe5770cd0772f20a8c9 |
| publicationDate | 2014-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8664355-B2 |
| titleOfInvention | Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor |
| abstract | It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract of Escherichia coli cells cultured at any temperature between 18° C. and 36° C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred. |
| priorityDate | 2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 508.