| abstract |
It is intended to provide a cell-free protein synthesis system with the use of a Xenopus oocyte extract, which is superior in protein synthesis efficiency to the microinjection method into oocytes or the existing method with the use of an egg extract. Namely, a kit for use in a cell-free protein synthesis comprising an extract prepared from Xenopus oocytes at the early stage of maturation entered from the state arrested at the first meiotic prophase in the cell cycle, an energy regenerating system, and at least one amino acid. |