| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ca2e4410855da087e11bf3eea8933fa |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-948 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate |
1989-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1993-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a9d7f5a005710aa043ae116fa06acd4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97fca77a1fe9936b00ca1a35d2c77dd7 |
| publicationDate |
1993-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5264341-A |
| titleOfInvention |
Selective cloning for high monoclonal antibody secreting hybridomas |
| abstract |
The invention is a method for selectively isolating cells which secrete higher levels of monoclonal antibody than did the parental culture from which they were derived. The method is based on the membrane characteristics of the cell and requires the use of a cell sorter. At present, the invention's best mode utilizes fluorescent probes in conjunction with a fluorescence activated cell sorter. The method is based on the correlation of a high level of cell surface immunoglobulin with high monoclonal antibody secretion rates. The invention provides a predictable and rapid process for selecting high monoclonal antibody producing cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5843657-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5843644-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003162947-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11618777-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8603774-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007243564-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007207539-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02066618-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8912385-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6784552-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8226976-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010291189-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8445232-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006008871-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2002253981-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7074407-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7629171-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6204030-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6867038-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006148012-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007224635-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7148040-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010287632-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8664355-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7993864-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009317862-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6569639-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008032329-A1 |
| priorityDate |
1989-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |