http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8058448-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a8a1cb36c35065d98f072e3cf942055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_20827b7b77fc1bf752f9ff8dcaf7df1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6abb2e6a85f530c6509d845d1784fa9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7e1bf4c88cbce1c59900f00c78b7e1b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a6b3b5d7b5da5a01533fffdb25d8aeb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_661b3ea63e73acc96731eb73625b1bc2 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D285-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D285-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate | 2009-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5430c6bb4fa95098c4f969390c120a44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2a347bbe63d275561b447447a5d9f97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5883858d7f09da3cbb401ab69f2793a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_436651b736dc6391c7c5c47e0567ff9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a03ad8811fc808b5ec37ce5323601a3f |
| publicationDate | 2011-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8058448-B2 |
| titleOfInvention | Processes and reagents for sulfurization of oligonucleotides |
| abstract | The present invention relates to processes and reagents for oligonucleotide synthesis and purification. One aspect of the present invention relates to compounds useful for activating phosphoramidites in oligonucleotide synthesis. Another aspect of the present invention relates to a method of preparing oligonucleotides via the phosphoramidite method using an activator of the invention. Another aspect of the present invention relates to sulfur-transfer agents. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to a method of preparing a phosphorothioate by treating a phosphite with a sulfur-transfer reagent of the invention. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to compounds that scavenge acrylonitrile produced during the deprotection of phosphate groups bearing ethylnitrile protecting groups. In a preferred embodiment, the acrylonitrile scavenger is a polymer-bound thiol. Another aspect of the present invention relates to agents used to oxidize a phosphite to a phosphate. In a preferred embodiment, the oxidizing agent is sodium chlorite, chloramine, or pyridine-N-oxide. Another aspect of the present invention relates to methods of purifying an oligonucleotide by annealing a first single-stranded oligonucleotide and second single-stranded oligonucleotide to form a double-stranded oligonucleotide; and subjecting the double-stranded oligonucleotide to chromatographic purification. In a preferred embodiment, the chromatographic purification is high-performance liquid chromatography. |
| priorityDate | 2004-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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