http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6329147-B1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b906c03bfe13e887fc19ee20cf246c56 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
filingDate | 2000-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2001-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c287e3901b9827cc0bd7078fbda54ebe |
publicationDate | 2001-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-6329147-B1 |
titleOfInvention | Methods for detection of a triplet repeat block and a functional mismatch binding protein in a biological fluid sample |
abstract | A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of an immobilized DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, including the presence of a mutated oncogene and the presence of DNA containing triplet repeat sequences which characterize several genetic diseases including fragile X syndrome. The present method is used to isolate or remove by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR-amplified DNA samples. Methods for detecting and enriching minority sequences are disclosed. Also provided are compositions and kits useful for practicing the methods of the present invention. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8105780-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2468885-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005191636-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005164236-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007084568-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2025762-A2 |
priorityDate | 1993-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1818.