| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78587acbfc8d4c9e9ac3baa7021087d7 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate |
1989-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_553cfcc7620722b0ad92299e79a7c788
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad9241240ee99474d84170fd12dc67bb |
| publicationDate |
1989-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0333465-A2 |
| titleOfInvention |
Mutation detection by competitive oligonucleotide priming |
| abstract |
The present invention relates to a process for the rapid and simple detection of mutations in DNA and differences between DNA sequences. This competitive oligonucleotide priming system can be used for the detection of any differences between DNA sequences for which a DNA sequence is known. n In its basic form the invention comprises the steps of:- nadding competitive oligonucleotide primers to a sample of nucleic acid or mixture of nucleic acids, wherein said competitive oligonucleotide primers include at least two primers, one being substantially complementary to the specific known sequence and at least one having a base mismatch with the specific known sequence; npreferentially hybridizing the substantially complementary primer to the specific known sequence under competitive conditions; nextending the preferentially hybridized primer from its 3′ terminus to synthesize an extension product complementary to the strand to which the primer is hybridized; and identifying said extension product. n Preferably the method includes the further steps of:- nadding a common oligonucleotide primer prior to said identifying step; nseparating said extension product from its complementary strand; and nrepeating said preferentially hybridizing and said extending steps. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6294336-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6033854-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8722328-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5567583-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5348853-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9918237-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5712386-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6316198-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6270967-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9113075-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9113075-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7026117-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6329147-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9885092-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9797000-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0149886-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9605303-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5605794-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2208797-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6013431-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2308997-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9689047-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6537748-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9626291-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0149886-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115410-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6225094-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9201811-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2551356-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2551356-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9114789-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7132235-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5137806-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0530794-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5728530-A |
| priorityDate |
1988-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |