| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26004d4f929d4468a3281047e0e1b29b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7c227edbc546f44c8030a6c1e6ff620 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8773
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-54 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-877
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 |
| filingDate |
2009-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08299e84c38f48987998d90bd2190e36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_263a73e38c305350de064a75166f61de |
| publicationDate |
2010-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2010146654-A1 |
| titleOfInvention |
Unactivated oocytes as cytoplast recipients for nuclear transfer |
| abstract |
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the GO or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit. |
| priorityDate |
1995-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |