http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7432415-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5943ac0d8098f07223d8ea739232b387 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8773 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-877 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 |
filingDate | 2005-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2008-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_971803fffa2f18ba77dd62531f631b04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd1e1b86c497f2bedac35f318cf4d412 |
publicationDate | 2008-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7432415-B2 |
titleOfInvention | Unactivated oocytes as cytoplast recipients for nonprimate mammalian and pig nuclear transfer |
abstract | A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G 0 or G 1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010146654-A1 |
priorityDate | 1995-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.