| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5943ac0d8098f07223d8ea739232b387 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8773
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-877
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate |
2004-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2008-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_971803fffa2f18ba77dd62531f631b04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd1e1b86c497f2bedac35f318cf4d412 |
| publicationDate |
2008-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7321076-B2 |
| titleOfInvention |
Unactivated ungulate oocytes to produce a cloned ungulate by nuclear transfer |
| abstract |
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G 0 or G 1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010146654-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009038020-A1 |
| priorityDate |
1995-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |