http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004053424-A1

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filingDate 2001-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cda3be1630d7b9311a1676f59c4379b6
publicationDate 2004-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004053424-A1
titleOfInvention Continuous-flow homogeneous biochemical assays based on mass spectrometric detection of known ligands
abstract The present invention relates to the measurement of interactions, and especially biospecific interactions in an assay using mass spectrometry. More in particular, the present invention makes it possible to measure specific interactions in a homogeneous biochemical assay. The on-line detection method of the present invention comprises an effluent of a fractionation step with a controlled amount of affinity molecules, which affinity molecules bind with analytes suspected to be present in the effluent. This step is followed by the addition of a controlled amount of known ligands, which ligands bind to the affinity molecules, by which ligand/affinity molecule-complexes are formed. In accordance with the invention, the free ligands are detected in the presence of the ligand/affinity molecule-complexes, using a mass spectrometer.
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priorityDate 2000-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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