| abstract |
A method for immobilizing amino-group containing molecules onto surfaces and devices having immobilized isocyanate-group containing molecules prepared by the method are disclosed. The method comprises reacting a surface (i.e., glass surface) having free hydroxyl groups with a silyl isocyanate derivatizing agent to provide immobilized reactive moieties, the agent having a formula: n (R 1 O)(R 2 O)(R 3 O)Si—X—NCO n wherein R 1 , R 2 and R 3 are independently represents C 1 -C 6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy; X represents linear or branched C 1 -C 20 alkyl or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, and sulfur and reacting the immobilized reactive moieties with the amino group-containing molecule so as to immobilize said molecule on the surface. Devices having a surface with immobilized molecules such as nucleic acids or proteins are useful for detection of target analytes in a sample. |