| abstract |
An indirect method for the quantitation of an antigen or antibody in a liquid sample. In the antigen assay, antigen of the same type as the sample antigen is adsorbed onto a solid support surface. Then, specific labelled antibody is immunologically reacted in solution with the sample antigen and the adsorbed antigen. The quantity of reacted labelled antibody on the solid support surface is then determined, as for example, fluorogen label by a fluorometer. The procedure is employed for detection of antibody by reversing the antigen and antibody components in each instance. |