abstract |
A device is disclosed for immobilization of proteins which includes a hydrophobic polymeric layer, preferably PVDF, attached to a rigid support. The proteins are spotted onto the dry surface of the PVDF. In preferred embodiments, the PVDF-coated rigid support advantageously provides greater ease of handling, protein immobilization in the dry state, high spot density, low background and high dynamic range. Methods of using the disclosed microarray support are also disclosed. |