| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_087bed7abab91ae0f927879f9fe8b273 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate |
2001-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f7fd163420706d3b47b8963dffe91bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_520c9dc6bcac212fce3d13550201a335
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b948abb07d25e9678c2e1836326b6d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84d4df7297eec7d674f42efaf6e2f02d |
| publicationDate |
2001-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2001055786-A1 |
| titleOfInvention |
Screening method for the discovery and directed evolution of oxygenase enzymes |
| abstract |
The present invention relates to screening methods for oxidation enzymes, particularly mono- and dioxygenases. According to the methods of the invention, a product of an oxidation reaction is converted into a phenol or a catechol, which is easily detected by a Gibbs assay. This conversion allows for a sensitive and efficient assay. Both high-throughput liquid-phase and solid-phase methods using these principles are provided. Also described are method for detecting phenolic ether-products and sulfhydryl products from oxidation reactions, also using a Gibbs assay. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021102933-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016003858-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10591496-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9897619-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016511828-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011124520-A1 |
| priorityDate |
2000-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |