Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc8f812f5002fd31552d82ad30daeb6b |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
filingDate |
2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2022-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef532f3b01b144150128088bd415df90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfca0ed80a28df7f5713970f68e55ee6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8ab323e9a59216c351ed0e3665dab7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1bf2d5a63fad428a11e0a2480a1d5cd |
publicationDate |
2022-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-11421259-B2 |
titleOfInvention |
Cell-free protein expression using rolling circle amplification product |
abstract |
Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system. |
priorityDate |
2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |