http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101103122-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b6c57c32ab97509a73c63ac366bc8296 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2005-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e68beecd3c998462f7787030d32d959d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4c8491e7ea66238493765ea134f6106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_496e86768fc37b7abf1c128db9a92701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3aa131820d613bd8a5b8d8bab30f2b2a |
| publicationDate | 2008-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101103122-A |
| titleOfInvention | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| abstract | The invention provides an improved cell free amplification method capable of producing large quantities of therapeutic-quality nucleic acids and methods of using the synthesized nucleic acid in research, therapeutic and other applications- The methods combine several different state-of-the-art procedures and coordinate their applications to affordably synthesize nucleic acids for therapeutic purposes. It combines in vitro rolling circle amplification, high fidelity polymerases, high affinity primers, and streamlined template specifically designed for particular applications. For expression purposes, the templates contain an expression cassette including a eukaryotic promoter, the coding sequence for the gene of interest, and a eukaryotic termination sequence. Following amplification, concatamers are subsequently processed according to their intended use and may include: restriction enzyme digestion for the production of short expression cassettes (SECs); ligation steps to circularize the SEC (CNAs); and/or supercoiling steps to produce sCNAs. The final product contains nearly non-detectable levels of bacterial endotoxin. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109072244-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019516368-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11421259-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103865922-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010005365-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103080337-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7058839-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103080337-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106676199-A |
| priorityDate | 2004-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 841.