http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008153128-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f59c9d7349cfe0bd7c9f8d7c73301e7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
| filingDate | 2007-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df46b3be21d998bbd4e1135b08512a7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d007c57af875546a425ab633103183c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c70e7ec0401be25f10a551688505734 |
| publicationDate | 2008-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2008153128-A1 |
| titleOfInvention | Cell-Free Protein Synthesis |
| abstract | The present invention relates to a method for synthesizing a protein in a cell-free synthesis system, which comprises the steps of: (a) amplifying a DNA molecule encoding the protein to prepare linear DNA molecules with a stem and loop sequence at their terminus, wherein the stem and loop sequence comprises a nucleotide sequence inducing the formation of a stem and loop structure at the 3′-end of transcripts of the amplified DNA molecules to prevent degradation of the transcripts; and (b) contacting the amplified DNA molecules to a lysate of cells with deficient endoribonuclease E activity, thereby producing the protein in the cell-free synthesis system. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10077459-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113801877-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11421259-B2 |
| priorityDate | 2006-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 481.