Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8381805eba6d034fc77bd230dd02e6e2 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2543-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2018-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84f0183dea199b424da6a0b2ddcc8ddc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_962187092ef81bac78c3e43b7c60b7bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6394f735878be53ae8422daeffb5aeef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04a2c717fd5cd024e14d1fa4de78e681 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8a683a44c230396dc57ecab98d9c466 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eea6a61db4a39b5829d7c5c05bd90bb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff4a0a21bf18520664df82c5bc83d856 |
publicationDate |
2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-10995361-B2 |
titleOfInvention |
Multiplexed signal amplified FISH via splinted ligation amplification and sequencing |
abstract |
The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023150163-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023150171-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4012046-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11739372-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021395796-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11781130-B2 |
priorityDate |
2017-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |