| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c97f90ab12b35b650d5fd039ed7a41a5 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate |
2013-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c377067715cac520aa70ac285942162
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ecdc3819d386ef4056671ce417ab264 |
| publicationDate |
2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2014235081-A |
| titleOfInvention |
Immunostaining method, fluorescent immunostaining pretreatment liquid and immunostaining kit |
| abstract |
The amount and the number of bright spots of fluorescent nanoparticles bound to an antigen in immunostaining using fluorescent nanoparticles for a pathological tissue fixed on a base glass treated with an amino-containing silane coupling agent Only provides a way to quantify. An immunostaining method for detecting an antigen in a pathological tissue fixed on the surface of a base glass treated with an amino-containing silane coupling agent by immunostaining using fluorescent nanoparticles, comprising: (i) A step of adding a blocking agent to the pathological tissue fixed on the surface of the base glass treated with the amino-containing silane coupling agent; and (ii) a step of adding an antibody that reacts with the antigen to the pathological tissue. And (iii) adding a compound (1) having a functional group capable of reacting with an amino group in the amino-containing silane coupling agent, and allowing to stand at room temperature for a predetermined time; (iv) the antibody; An immunostaining method comprising a step of adding fluorescent nanoparticles having a functional group capable of reacting and binding. [Selection figure] None |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11674954-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017026375-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018155601-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11674966-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018181795-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109085335-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190120327-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020073911-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190120326-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018181800-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7097396-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11733244-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11519860-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11486879-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10816469-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019515252-A |
| priorityDate |
2013-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |