| abstract |
Disclosed is a mutant luciferase enzyme having greatly enhanced thermal stability compared to natural or mutant luciferases. SOLUTION: Using a beetle luciferase gene, it is applied to various means of random mutagenesis, especially gene synthesis using polymerase which is prone to error, to make a modified luciferase gene library, to be expressed in E. coli, and to select mutations A combined modified luciferase was made. A new library is made from this complex modified luciferase by random mutagenesis and consists of regression mutagenesis that repeatedly selects this process. [Selection] Figure 1 |