abstract |
SUMMARY OF THE INVENTION A luciferase enzyme with greatly enhanced thermostability, eg, a half-life of at least 2 hours at 50 ° C., a cDNA encoding this novel luciferase, and transformed to express the luciferase. A host is disclosed. The method for producing luciferase comprises regression mutagenesis. This luciferase is used in a conventional manner, and a kit is partially used. |