| abstract |
(57) [Summary] [Structure] In the amino acid sequence of wild-type firefly luciferase, the amino acid at position 217, or the amino acid at the same position as position 217 in luciferase of Genji firefly or Heike firefly is mutated to a hydrophobic amino acid. A thermostable firefly luciferase, a gene encoding the thermostable luciferase, a vector incorporating the gene encoding the thermostable luciferase, and a method for producing a thermostable firefly luciferase using the vector. The thermostable firefly luciferase obtained by expressing the thermostable firefly luciferase gene of the present invention is A It is extremely useful in microassays such as TP. Further, by the method for producing a thermostable firefly luciferase of the present invention, It is possible to efficiently obtain the above luciferase, It is extremely useful in industry. |