| abstract |
The present invention provides a method for improving the incidence in somatic cell nuclear transfer technology and artificial insemination technology using sperm cells. A method for producing a nuclear transplanted egg, comprising a step of transplanting a nucleus of a donor cell into an egg, and a step of treating the egg transplanted with a nucleus with an antimethylating agent, and transplanting the nuclear transplanted egg into an animal To produce a cloned animal. The demethylating agent is a histone deacetylase inhibitor, and the donor cell is selected from cumulus cells, fibroblasts, and ES cells. [Selection figure] None |