Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2517-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0273 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-877 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1137 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate |
2015-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
2017-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2017528142-A |
titleOfInvention |
Methods and compositions for increasing somatic cell nuclear transfer (SCNT) efficiency by eliminating histone H3-lysine trimethylation |
abstract |
The present invention provides methods and compositions for improving the efficiency of somatic cell nuclear transfer (SCNT), and the resulting nuclear transfer ESC (ntESC) and generation of transgenic cells and / or transgenic non-human animals. More specifically, the present invention demonstrates that histone H3-lysine 9 trimethylation (H3K9me3) in the reprogramming resistance region (RRR) in nuclear genetic material of donor somatic cells is an efficient somatic cell nuclear reprogramming or SCNT. Concerning the discovery of blocking. The present invention improves the efficacy of SCNT by exogenous expression of the demethylase Kdm4 family, ie, overexpression, and / or by inhibiting methylation of H3K9me3 by inhibiting histone methyltransferases Suv39h1 and / or Suv39h2 Methods and compositions for reducing H3K9me3 are provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7199741-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021514199-A |
priorityDate |
2014-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |