| abstract |
We provide (a) (i) a support medium; and (ii) providing a reference standard or a planar section thereof containing an amount of detectable entity supported by the support medium, wherein the detectable entity is Has an elongate path and a predetermined amount of detectable entity is present in a defined region of the reference standard cross-section; (b) the presence of a detectable entity in the reference standard, its planar section, or the defined region, or Obtaining a first reference signal indicative of the amount; (c) providing a biological sample and second indicating the presence or amount of the detectable entity in the biological sample, a planar section thereof, or a defined area. And (d) comparing the reference signal obtained in (b) against the second signal obtained in (c). Preferably, the method is used to indicate the presence, amount or concentration of a detectable entity in a biological sample. |